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KMID : 0613820040140050815
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Journal of Life Science
2004 Volume.14 No. 5 p.815 ~ p.820
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Antitumor activity of Bacillus subtilis SW-1 isolated from Jeotgal
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Park Jong-Kia
Cho Yong-Un
Choi Young-Ju
Jeong Yong-Kee
Gal Sang-Wan
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Abstract
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A bacterum containing antitumor activity was isolated from traditional korean food, Jeotgal. Through the 16s rRNA sequence analysis, the bacterium was identitied as a strain of Bacillus subtilis SW-1. The best culture condition for antitumor activity of the bacterium is 3% of soluble starch and 1% of yeast extract as corbon and nitrogen sources, respcctively. Cytotoxicitic concentrations of the culture supernatant of B. subtilis SW-1 against cancer cell lines, A549 and SK-OV3 were 30 μl/ml and 40 μl/ml, respectively, as IC50 values. In DNA fragmentation assay, the culture supernatant showed the programmed cell death (apoptosis) to cause degrading the chromosomal DNA like ladder. Taken together, the culture supernatant of the B. subtilis SW-1 has some possibility to be used as an antitumor agent.
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KEYWORD
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Jeotgal, Bacillus subtilis, antitumor, apoptosis
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